高级搜索
孟令娇, 丁平安, 巨英超, 刘思桦, 常胜, 韩若琦, 潘烁, 刘飞, 谷丽娜, 桑梅香. 环状RNA ciRS-7在三阴性乳腺癌中的表达及其对细胞侵袭和迁移的影响[J]. 肿瘤防治研究, 2019, 46(3): 233-238. DOI: 10.3971/j.issn.1000-8578.2019.18.0855
引用本文: 孟令娇, 丁平安, 巨英超, 刘思桦, 常胜, 韩若琦, 潘烁, 刘飞, 谷丽娜, 桑梅香. 环状RNA ciRS-7在三阴性乳腺癌中的表达及其对细胞侵袭和迁移的影响[J]. 肿瘤防治研究, 2019, 46(3): 233-238. DOI: 10.3971/j.issn.1000-8578.2019.18.0855
shu
MENG Lingjiao, DING Ping'an, JU Yingchao, LIU Sihua, CHANG Sheng, HAN Ruoqi, PAN Shuo, LIU Fei, GU Li'na, SANG Meixiang. Expression of Circular RNA ciRS-7 and Its Effect on Invasion and Migration of Triple-negative Breast Cancer Cells[J]. Cancer Research on Prevention and Treatment, 2019, 46(3): 233-238. DOI: 10.3971/j.issn.1000-8578.2019.18.0855
Citation: MENG Lingjiao, DING Ping'an, JU Yingchao, LIU Sihua, CHANG Sheng, HAN Ruoqi, PAN Shuo, LIU Fei, GU Li'na, SANG Meixiang. Expression of Circular RNA ciRS-7 and Its Effect on Invasion and Migration of Triple-negative Breast Cancer Cells[J]. Cancer Research on Prevention and Treatment, 2019, 46(3): 233-238. DOI: 10.3971/j.issn.1000-8578.2019.18.0855
shu

环状RNA ciRS-7在三阴性乳腺癌中的表达及其对细胞侵袭和迁移的影响

Expression of Circular RNA ciRS-7 and Its Effect on Invasion and Migration of Triple-negative Breast Cancer Cells

    摘要
  • 摘要:
    目的 探讨环状RNA ciRS-7在三阴性乳腺癌（TNBC）中的表达及其对细胞侵袭和迁移的影响。
    方法 通过qRT-PCR法检测乳腺癌组织和细胞中ciRS-7的表达水平，分析其与乳腺癌患者临床病理指标之间的关系。用划痕实验和Transwell实验检测沉默ciRS-7后MDA-MB-231和BT-549细胞的迁移和侵袭能力的变化。利用动物实验在体内进行验证。
    结果 TNBC组织中ciRS-7的相对表达量为（6.52±0.38），显著高于Luminal型（1.56±0.17）（P < 0.001）和HER2过表达型乳腺癌组织（2.27±0.66）（P < 0.001）以及癌旁正常组织（0.83±0.09）（P < 0.001）。ciRS-7表达与分子分型、肿瘤浸润和淋巴结转移明显相关（均P < 0.05）；而与患者年龄、肿瘤直径和病理分级无关（均P > 0.05）。沉默ciRS-7后，MDA-MB-231和BT-549细胞的迁移和侵袭明显减弱（均P < 0.05）。ciRS-7敲低组肺转移瘤数明显减少（P < 0.05）。
    结论 ciRS-7在TNBC中高表达，并可能增强TNBC细胞的侵袭和迁移。

     

    Abstract:
    Objective To investigate the expression of circular RNA ciRS-7 in triple-negative breast cancer (TNBC) and its effect on invasion and migration.
    Methods The expression levels of ciRS-7 in TNBC tissues and cells were detected by qRT-PCR, and its relationship with clinicopathological parameters of breast cancer patients was further analyzed. Scratch and Transwell assays were used to detect the migration and invasion of MDA-MB-231 and BT-549 cells after silencing ciRS-7. Animal experiment was performed to verify the above findings in vivo.
    Results The relative expression of ciRS-7 in TNBC tissues was (6.52±0.38), significantly higher than Luminal type (1.56±0.17) (P < 0.001), HER2 overexpressing breast cancer tissues (2.27±0.66) (P < 0.001) and normal adjacent tissues (0.83 ± 0.09) (P < 0.001). Clinical data analysis showed that ciRS-7 expression was significantly associated with molecular typing, tumor invasion and lymph node metastasis (all P < 0.05), not associated with patients' age, tumor diameter or pathological grade (all P > 0.05). After silencing ciRS-7, the migration and invasion of MDA-MB-231 and BT-549 cells were significantly reduced (all P < 0.05). The number of lung metastasis clones was significantly reduced in ciRS-7 knockdown group (P < 0.05).
    Conclusion ciRS-7 is highly expressed in TNBC and may enhance the invasion and migration of TNBC cells.

     

    • HTML全文
    • 参考文献(23)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回

This work is licensed under a Creative Commons Attribution 3.0 License.

邮件订阅 RSS
总访问量： 今日访问：