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龚芬芬, 韩鹏黎, 崔渊博, 曹新广, 赵瑞华, 展翔, 李学民, 曹巍. 微小RNA-181d在食管鳞状细胞癌中的表达及其临床意义[J]. 肿瘤防治研究, 2018, 45(12): 981-984. DOI: 10.3971/j.issn.1000-8578.2018.18.0716
引用本文: 龚芬芬, 韩鹏黎, 崔渊博, 曹新广, 赵瑞华, 展翔, 李学民, 曹巍. 微小RNA-181d在食管鳞状细胞癌中的表达及其临床意义[J]. 肿瘤防治研究, 2018, 45(12): 981-984. DOI: 10.3971/j.issn.1000-8578.2018.18.0716
GONG Fenfen, HAN Pengli, CUI Yuanbo, CAO Xinguang, ZHAO Ruihua, ZHAN Xiang, LI Xuemin, CAO Wei. Expression and Clinical Significance of miRNA-181d in Esophageal Squamous Cell Carcinoma[J]. Cancer Research on Prevention and Treatment, 2018, 45(12): 981-984. DOI: 10.3971/j.issn.1000-8578.2018.18.0716
Citation: GONG Fenfen, HAN Pengli, CUI Yuanbo, CAO Xinguang, ZHAO Ruihua, ZHAN Xiang, LI Xuemin, CAO Wei. Expression and Clinical Significance of miRNA-181d in Esophageal Squamous Cell Carcinoma[J]. Cancer Research on Prevention and Treatment, 2018, 45(12): 981-984. DOI: 10.3971/j.issn.1000-8578.2018.18.0716

微小RNA-181d在食管鳞状细胞癌中的表达及其临床意义

Expression and Clinical Significance of miRNA-181d in Esophageal Squamous Cell Carcinoma

  • 摘要:
    目的 探讨miR-181d在食管鳞状细胞癌组织中的表达,并分析其临床意义。
    方法 应用反转录实时荧光定量PCR(reverse transcription quantitative real-time PCR, RT-qPCR)技术检测37例食管鳞状细胞癌癌组织及癌旁组织中miR-181d的表达,分析miR-181d表达与肿瘤临床病理特征的相关性。
    结果 miR-181d在89.2%(33/37)的食管鳞状细胞癌组织中表达水平显著高于对应癌旁组织(P < 0.01);miR-181d表达水平与性别、年龄、浸润深度、淋巴结转移、组织学分级、肿瘤部位均无明显相关性(P > 0.05),但与TNM分期显著相关(P < 0.05)。Kaplan-Meier生存分析显示,miR-181d高表达组总生存率和无病生存率明显低于低表达组(P < 0.05)。
    结论 miR-181d在食管鳞状细胞癌组织中的表达与肿瘤的TNM分期及预后相关,miR-181d可能作为潜在预测指标评估食管鳞状细胞癌患者的预后。

     

    Abstract:
    Objective To observe the expression of microRNA(miRNA, miR)-181d in esophageal squamous cell carcinoma (ESCC) tissues and to explore its clinical significance.
    Methods Reverse transcription quantitative real-time PCR(RT-qPCR) was used to detect the miR-181d expression in 37 cases of ESCC tissues and matched adjacent tissues. The correlations of miR-181d expression with clinicpathological features and clinical prognosis were analyzed.
    Results miR-181d expression was up-regulated in 89.2%(33/37) of ESCC tissues, compared with the matched adjacent tissues (P < 0.01). The expression level of miR-181d was not significantly correlated with gender, age, depth of invasion, lymph node metastasis, histological grade or tumor location(P > 0.05), but significantly related to TNM staging (P < 0.05). Kaplan-Meier survival analysis showed that the overall survival rate and the disease-free survival rate of the high miR-181d expression group was significantly lower than that of the low miR-181d expression group (P < 0.05).
    Conclusion miR-181d expression is related to TNM and prognosis of ESCC patients. miR-181d may be used as a potential predictor to evaluate the prognosis of ESCC patients.

     

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