Garcinone E抑制鼻咽癌细胞增殖、迁移和侵袭能力的研究

孔晓阳; 刘夏; 董彩华; 夏玉对; 卢奕; 张健

doi:10.3971/j.issn.1000-8578.2018.18.0161

Garcinone E抑制鼻咽癌细胞增殖、迁移和侵袭能力的研究

Garcinone E Inhibits Proliferation, Migration and Invasion of Human Nasopharyngeal Carcinoma Cells

摘要

摘要:
目的探讨源于岭南山竹的天然产物单体Garcinone E对鼻咽癌细胞增殖、迁移侵袭能力的影响及其分子机制。
方法 MTS法检测鼻咽癌HONE1和HK1细胞的增殖率；Transwell细胞小室检测HONE1和HK1细胞迁移和侵袭的能力；Western blot和q-PCR法检测HONE1和HK1细胞上皮间充质转化（EMT）相关标志性蛋白和基因表达及可能的信号通路。
结果 MTS 24 h结果显示Garcinone E可显著抑制鼻咽癌细胞HONE1和HK1的生长，并和浓度呈正相关作用。Transwell小室实验显示，与对照组相比，Garcinone E的给药浓度为7.5 μmol/L时，HONE1和HK1细胞穿过小室的数量明显减少，其迁移和侵袭能力明显受到抑制。Western blot检测发现Garcinone E呈剂量依赖性地下调p-Vimentin、Snail及Slug蛋白的表达，同时增加E-cadherin蛋白的表达。q-PCR表明Garcinone E呈剂量依赖性地上调两种鼻咽癌细胞中E-cadherin的mRNA表达。
结论 Garcinone E能够有效抑制鼻咽癌细胞增殖并抑制鼻咽癌细胞迁移和侵袭能力，其机制可能与抑制鼻咽癌细胞EMT的发生有关。

Abstract:
Objective To explore the effect of Garcinone E, extracted from Garcinia oblongifolia Champ, on the proliferation, migration and invasion of nasopharyngeal carcinoma(NPC) HK1 and HONE1 cells and elucidate its possible mechanism.
Methods The effect of Garcinone E on the proliferation, migration and invasion of nasopharyngeal carcinoma HK1 and HONE1 cells lines was examined using MTS and Transwell assay, respectively. The expressions of EMT-related protein and mRNA and possible pathway were detected by Western blot and q-PCR.
Results MTS assay showed that Garcinone E significantly inhibited the viability of HK1 and HONE1 cell lines in a dose-dependent manner. Transwell assay showed that compared with the blank control group, 7.5μmol/L Garcinone E suppressed the migration and invasion of HK1 and HONE1 cell lines obviously. Western blot results indicated that Garcinone E decreased the expression of p-Vimentin, Snail and Slug and increased the expression of E-cadherin in a dose-dependent manner. q-PCR assay showed that Garcinone E increased the expression of E-cadherin mRNA in HK1 and HONE1 cell lines.
Conclusion Garcinone E could inhibit the proliferation, migration and invasion abilities of nasopharyngeal carcinoma cells, which may be related to regulating the process of EMT.

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