miR-130b调控RAB34的表达在胰腺癌发生发展中的作用

何峰; 陈曦; 程方雄; 闵小春; 曹忠厚; 丁锋

doi:10.3971/j.issn.1000-8578.2018.17.0960

miR-130b调控RAB34的表达在胰腺癌发生发展中的作用

Effect of miR-130b Targeting RAB34 Expression in Occurrence and Development of Pancreatic Cancer

摘要

摘要:
目的探讨miR-130b调控Ras相关蛋白34（Ras-related proteins in brain 34, RAB34）在胰腺癌中的表达。
方法收集22例胰腺癌及其癌旁组织标本，采用免疫组织化学、Western blot和qRT-PCR法检测RAB34的表达；qRT-PCR法检测miR-130b表达；细胞转染法检测miR-130b调控RAB34表达，双荧光素酶报告基因检测系统验证miR-130b结合RAB34的3’UTR的靶位点。
结果与癌旁组织对比，RAB34在胰腺癌组织中显著高表达，且miR-130b显著低表达；转染miR-130b mimics可抑制RAB34的表达；RAB34是miR-130b作用的靶基因。
结论 RAB34是胰腺癌的分子标志物，miR-130b可调控其表达。

Abstract:
Objective To investigate the expression of Ras-related proteins in brain 34 (RAB34) regulated by miR-130b in pancreatic cancer.
Methods We collected 22 paired specimens of primary pancreatic cancer and adjacent normal tissues specimens. Immunohistochemistry, Western blot and qRT-PCR were employed to detect the expression level of RAB34. Meanwhile, the expression of miR-130b was detected by qRT-PCR. Cell transfection method was used to discover the regulation of miR-130b on RAB34 gene. The luciferase reporter assay was applied to identify whether RAB34 was directly targeted by miR-130b.
Results Compared with adjacent tissues specimens, miR-130b was significantly downregulated and its target gene RAB34 was upregulated in pancreatic cancer tissues. Overexpression of miR-130b remarkably inhibited the expression of RAB34. Moreover, the dual luciferase assay revealed that RAB34 was directly targeted by miR-130b.
Conclusion RAB34 might have a considerable potential in prognosis identification of pancreatic cancer and be regulated by miR-130b.

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