乳腺癌组织中PDK4的表达及其与预后的关系

孙书娟; 赵美松; 韩迎燕; 吴娟子; 吴鹏; 刘佳

doi:10.3971/j.issn.1000-8578.2018.17.0737

乳腺癌组织中PDK4的表达及其与预后的关系

PDK4 mRNA Expression in Breast Cancer and Its Relationship with Prognosis

摘要

摘要:
目的探讨影响乳腺癌组织中PDK4的表达与预后的相关性。
方法 PubMed数据库The Gene Expression Omnibus（GEO, http://www.ncbi.nlm.nih.gov/geo/）分析PDK4在正常乳腺及乳腺癌组织中的表达水平。使用The Kaplan–Meier plotter（www.kmplot.com）分析工具评估PDK4表达水平与乳腺癌临床预后的相关性。TGF-β细胞因子处理MCF-7细胞系，Real-time PCR技术检测经处理后MCF-7细胞系中PDK4 mRNA表达水平。siRNA干扰技术降低细胞内PDK4的表达水平，Transwell检测MCF-7细胞的侵袭能力。
结果 PDK4在乳腺癌组织中的表达水平低于正常乳腺组织，并且PDK4低表达与乳腺癌预后差呈正相关；在乳腺癌细胞系中，TGF-β降低PDK4 mRNA表达水平，且PDK4的低表达增强了MCF-7细胞的侵袭能力。
结论 PDK4低表达是乳腺癌预后差的一个重要指标。

Abstract:
Objective To explore PDK4 mRNA expression in breast cancer tissues and its relationship with prognosis.
Methods We analyzed the mRNA expression of PDK4 by the Gene Expression Omnibus (GEO, http://www.ncbi.nlm.nih.gov/geo/) and estimated the correlation of PDK4 mRNA expression with the prognosis of breast cancer patients by Kaplan-Meier plotter (www.kmplot.com). Real-time PCR was used to detect the mRNA expression of PDK4 in TGF-β-treated MCF-7 cell line. siRNA decreased the mRNA level of PDK4 in breast cancer cells. Transwell assay was used to detect the invasion ability of MCF-7 cells.
Results PDK4 was down-regulated in breast cancer tissues, and the low expression of PDK4 was positively correlated with poor prognosis of breast cancer patients. In breast cancer cell lines, TGF-β decreased PDK4 mRNA expression, and the low expression of PDK4 enhanced the invasion of MCF-7 cells.
Conclusion The low mRNA level of PDK4 is an important prognosis indicator for breast cancer.

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