石蒜碱诱导肺癌NCI-H460细胞凋亡作用及其机制

李居伟; 杨扬

doi:10.3971/j.issn.1000-8578.2018.17.0388

石蒜碱诱导肺癌NCI-H460细胞凋亡作用及其机制

李居伟,
杨扬

Apoptosis Induction of Lycorine on Lung Cancer NCI-H460 Cells and Related Mechanism

LI Juwei,
YANG Yang

摘要

摘要:
目的通过研究石蒜碱在体外对人肺癌NCI-H460细胞增殖抑制和诱导凋亡的作用，探讨石蒜碱抗肿瘤机制。
方法 MTT法检测石蒜碱对NCI-H460细胞增殖的抑制作用，流式细胞术检测NCI-H460细胞的凋亡率，比色法检测凋亡因子相关因子Caspase 3活性。
结果石蒜碱能够有效抑制肺癌NCI-H460细胞增殖，IC₅₀为（5.79±0.11）μmol/L，对NCI-H460细胞的凋亡具有诱导效应，可增强肺癌Caspase 3的活性。
结论石蒜碱在体外能有效地抑制NCI-H460细胞增殖并促进其凋亡，其机制可能与激活Caspase 3的表达从而诱导肿瘤细胞凋亡有关。

Abstract:
Objective To investigate the effect of lycorine on the proliferation inhibition and apoptosis induction in human lung cancer NCI-H460 cells, and to explore the anti-tumor mechanism of lycorine.
Methods MTT assay was used to detect the proliferation of NCI-H460 cells. The apoptosis rate of NCI-H460 cells was detected by flow cytometry. The activity of Caspase 3 was detected by colorimetry.
Results Lycorine could inhibit the proliferation of NCI-H460 cells, and the IC₅₀ was (5.79±0.11)μmol/L. Lycorine had an induced effect on the apoptosis of NCI-H460 cells. Lycorine could enhance Caspase 3 activity.
Conclusion Lycorine could effectively inhibit the proliferation and induce the apoptosis of NCI-H460 cells in vitro, and its mechanism may be related to the activation of Caspase 3 expression and the apoptosis induce of tumor cells.

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