芦丁联合奥沙利铂激活FKN/SYK/p38通路促进胃癌细胞凋亡

李琪; 任立群; 王亚帝; 陈素贤

doi:10.3971/j.issn.1000-8578.2017.17.0603

芦丁联合奥沙利铂激活FKN/SYK/p38通路促进胃癌细胞凋亡

Effect of Rutin Combined with Oxaliplatin on Activation of FKN/SYK/p38 Pathway to Promote Apoptosis of Gastric Cancer Cells

摘要

摘要:
目的探讨芦丁联合奥沙利铂对人胃癌细胞SGC-7901增殖和凋亡的影响及机制。
方法采取对数生长期的人胃癌细胞SGC-7901作为研究对象，采用MTT、流式细胞术、Western blot等方法检测细胞活力、细胞周期和相关蛋白表达的变化。
结果 p38抑制剂可促进SGC-7901细胞的增殖（P < 0.05）；而芦丁、奥沙利铂均可抑制SGC-7901细胞的增殖，联合组对SGC-7901细胞的抑制作用更显著；p38抑制剂组FKN、SYK、p-p38、cleaved-caspase3、7、8、9蛋白表达与阴性对照组相比明显降低（P < 0.05）；芦丁与奥沙利铂联合作用后，以上蛋白均不同程度的升高。
结论芦丁与奥沙利铂均可抑制SGC-7901细胞增殖并诱导其凋亡，其机制可能与FKN/SYK/p38通路的激活有关。

Abstract:
Objective To investigate the effect of rutin combined with oxaliplatin on the proliferation and apoptosis of human gastric cancer SGC-7901 cells and related mechanism.
Methods We took human gastric cancer SGC-7901 cells in the logarithmic growing period as the research object. MTT, flow cytometry and Western blot were used to detect cell viability, cell cycle and related protein expression.
Results P38 inhibitor could promote SGC-7901 cells proliferation(P < 0.05). Either rutin or oxaliplatin could inhibit the proliferation of SGC-7901 cells, and the inhibitory effect of their combination was more significant. The expressions of FKN, SYK, p-p38, cleaved-caspase3, cleaved-caspase7, cleaved-caspase8 and cleaved-caspase9 protein in p38 inhibitor group were significantly lower than those in negative control group(P < 0.05); the above proteins expressions were increased in varying degrees after treated with rutin combined with oxaliplatin.
Conclusion Rutin and oxaliplatin could inhibit the proliferation and induce apoptosis of SGC-7901 cells. The mechanism may be related to the activation of FKN/SYK/p38 pathway.

HTML全文

参考文献(18)

施引文献

资源附件(0)