金丝桃苷诱导人胃癌细胞MKN-45凋亡及其机制

刘海雯; 郭莲怡

doi:10.3971/j.issn.1000-8578.2017.17.0461

金丝桃苷诱导人胃癌细胞MKN-45凋亡及其机制

Hyperoside Induces Apoptosis of Gastric Cancer Cell Line MKN-45 and Related Mechanism

摘要

摘要:
目的探讨金丝桃苷（hyperoside, Hyp）对人胃癌MKN-45细胞增殖及凋亡的影响及其机制。
方法体外培养MKN-45细胞，对照组不加药物，实验组分别加入不同浓度Hyp（12.5、25、50、100、200 μg/ml）作用24、48 h，MTT法检测Hyp对细胞增殖的影响并筛选适宜药物浓度；流式细胞术检测细胞周期变化及凋亡率；Western blot法分析NF-κB P65、caspase-3、Bax、Bcl-2蛋白的表达情况。
结果 Hyp可明显抑制MKN-45细胞的增殖，阻滞细胞于G₀/G₁期并促进其凋亡，Western blot结果显示，随着药物浓度的增高，Hyp可使NF-κB P65、Bcl-2蛋白表达降低，casepase-3、Bax蛋白表达增高。
结论金丝桃苷可抑制MKN-45细胞增殖并诱导凋亡，其机制可能与该药物阻断细胞周期，抑制NF-κB通路，下调NF-κB P65、Bcl-2蛋白，上调casepase-3、Bax蛋白有关。

Abstract:
Objective To investigate the effects of hyperoside(Hyp) on the proliferation and apoptosis of human gastric cell line MKN-45 and its mechanism.
Methods Gastric cells MKN-45 cultured in vitro were treated with various concentrations of hyperoside(0, 12.5, 25, 50, 100, 200μg/ml) for 24 and 48h. Cell proliferation was examined by MTT which could screen suitable drug concentration; cell cycle and apoptosis were analyzed by flow cytometry; NF-κB P65, casepase-3, Bcl-2 and Bax protein expressions were examined by Western blot.
Results Hyperoside could inhibit the growth of MKN-45 cells observably, it also blocked cell cycle at G₀/G₁ stage and induced cell apoptosis. Western blot showed that hyperoside could reduce the expression of NF-κB P65, Bcl-2 protein and elevate the expression of caspase-3, Bax protein with the increase of drug concentration.
Conclusion Hyperoside could inhibit the growth of MKN-45 cells and induce cell apoptosis. The mechanism might be related to blocking cell cycle, inhibiting NF-κB pathway, reducing the expression of NF-κB P65 and Bcl-2 protein and elevating the expression of caspase-3 and Bax protein.

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