小干扰RNA沉默<i>YKL</i>-<i>40</i>基因对子宫内膜癌HEC-1A细胞顺铂化疗敏感度的影响

李莉莉; 范江涛; 李大海; 刘炎

doi:10.3971/j.issn.1000-8578.2017.16.0658

小干扰RNA沉默YKL-40基因对子宫内膜癌HEC-1A细胞顺铂化疗敏感度的影响

Effects of siRNA Targeting YKL-40 Gene on Chemosensitivity of Endometrial Cancer HEC-1A Cells to Cisplatin

摘要

摘要:
目的通过siRNA沉默YKL-40基因探讨其对子宫内膜癌HEC-1A细胞顺铂化疗敏感度的影响。
方法 siRNA基因干扰技术沉默YKL-40基因，将人子宫内膜癌HEC-1A细胞分为三组：空白对照组、空载体组、siRNA实验组。qRT-PCR法检测沉默效率。qRT-PCR法检测顺铂处理子宫内膜癌细胞对癌细胞中YKL-40基因的影响。MTT法和流式细胞术检测靶细胞的增殖能力以及凋亡率。
结果转染组YKL-40 mRNA的表达量显著低于空白对照组和空载体组（P=0.036, P=0.005），经过相同浓度的顺铂处理子宫内膜癌后，YKL-40基因的表达量上调。转染组的增殖能力低于空白对照组和空载体组（P < 0.05），而凋亡率高于空白对照组和空载体组（P=0.000, P=0.000）。
结论 YKL-40基因沉默可以提高子宫内膜癌HCE-1A细胞对顺铂的敏感度。YKL-40基因具有抗凋亡作用。

Abstract:
Objective To explore the effects of silencing YKL-40 gene by small interfering RNA (siRNA) on the chemosensitivity of endometrial cancer HEC-1A cells to cisplatin.
Methods We silenced the YKL-40 gene in HEC-1A cells using the siRNA technique and divided the HEC-1A cells into three groups as blank control group, mock-treatment group and experimental group, respectively. The mRNA expression of YKL-40 was measured using quantitative real time-PCR(qRT-PCR) assay after treated with cisplatin. The proliferation and apoptosis of HEC-1A cells were also determined using MTT assay and flow cytometry(FCM) respectively.
Results The mRNA expression of YKL-40 in the experimental group was significantly lower than those in blank control and mock-treatment groups(P=0.036, 0.005, respectively). The mRNA expression of YKL-40 in HEC-1A cells was up-regulated after treatment of HEC-1A cells with the same concentration of cisplatin. The proliferative ability of HEC-1A cells was decreased(P < 0.05) while the apoptosis was increased(P=0.000) significantly in the experimental group, compared with the other two groups.
Conclusion The chemosensitivity of HCE-1A cells to cisplatin could be elevated by silencing YKL-40 gene. YKL-40 gene in HCE-1A cells have the ability of anti-apoptosis.

HTML全文

参考文献(29)

施引文献

资源附件(0)