度洛西汀对多西他赛化疗所致大鼠神经病理性疼痛的影响

彭平; 晁腾飞; 蒋继宗; 汪蕾; 于世英; 邱红

doi:10.3971/j.issn.1000-8578.2016.09.006

度洛西汀对多西他赛化疗所致大鼠神经病理性疼痛的影响

Effect of Duloxetine on Docetaxel-induced Neuropathic Pain in Rats

摘要

摘要:
目的研究度洛西汀对多西他赛化疗所致大鼠神经病理性疼痛的作用。
方法 24只SD雄性大鼠随机分为4组（n=6）：正常对照组、度洛西汀组、多西他赛模型组及度洛西汀治疗组。在治疗后第8天，检测大鼠的机械性痛阈、热痛阈、冷痛阈，以及L_4~6脊髓背根神经节（dorsal root ganglia, DRG）P物质（substance P, SP）的释放。
结果多西他赛能够产生机械性痛觉异常（P=0.009）、热痛觉过敏（P<0.001）、冷痛觉异常（P<0.001），以及L_4~6脊髓DRG的SP释放（P=0.018）。而连续8天口服度洛西汀能有效降低多西他赛导致的神经病理性疼痛，减轻机械性痛觉异常（P=0.037），热痛觉过敏（P=0.001）和冷痛觉异常（P=0.002），但并没有减少P物质的释放（P=0.653）。
结论度洛西汀可能是一种潜在的有效减轻化疗诱导神经病理性疼痛的药物。

Abstract:
Objective To investigate the efficacy of Duloxetine on docetaxel-induced neuropathic pain in rats.
Methods A total of 24 male Sprague-Dawley rats were randomly divided into four groups: naive control, Duloxetine control group, docetaxel group and Duloxetine treated group. On day 8, mechanical threshold, heat threshold, cold allodynia, and substance P(SP) released in L4-6 dorsal root ganglia(DRG) in the lumbar spinal cord were examined by RT-PCR.
Results Docetaxel could evoke mechanical allodynia(P=0.009), heat hypoalgesia(P<0.001) and cold allodynia(P<0.001), and increase the release of SP in L4-6 DRG, while the oral administration of Duloxetine for 8d significantly ameliorated mechanical allodynia(P=0.037), heat hypoalgesia(P=0.001), cold allodynia(P=0.002), without changing the release of SP(P=0.653).
Conclusion Duloxetine may be a potential treatment agent against chemotherapy-induced neuropathic pain.

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