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王秀存, 吴金霞, 布会敏, 杜文琪, 裴冬生. Rap2a对肺癌细胞侵袭能力的影响及其机制[J]. 肿瘤防治研究, 2016, 43(8): 668-672. DOI: 10.3971/j.issn.1000-8578.2016.08.004
引用本文: 王秀存, 吴金霞, 布会敏, 杜文琪, 裴冬生. Rap2a对肺癌细胞侵袭能力的影响及其机制[J]. 肿瘤防治研究, 2016, 43(8): 668-672. DOI: 10.3971/j.issn.1000-8578.2016.08.004
WANG Xiucun, WU Jinxia, BU Huimin, DU Wenqi, PEI Dongsheng. Effect of Rap2a on Invasion and Metastasis of Lung Cancer Cells and Its Related Mechanism[J]. Cancer Research on Prevention and Treatment, 2016, 43(8): 668-672. DOI: 10.3971/j.issn.1000-8578.2016.08.004
Citation: WANG Xiucun, WU Jinxia, BU Huimin, DU Wenqi, PEI Dongsheng. Effect of Rap2a on Invasion and Metastasis of Lung Cancer Cells and Its Related Mechanism[J]. Cancer Research on Prevention and Treatment, 2016, 43(8): 668-672. DOI: 10.3971/j.issn.1000-8578.2016.08.004

Rap2a对肺癌细胞侵袭能力的影响及其机制

Effect of Rap2a on Invasion and Metastasis of Lung Cancer Cells and Its Related Mechanism

  • 摘要:
    目的 检测小G蛋白家族成员Rap2a对人肺癌细胞迁移侵袭的影响及机制探讨。
    方法 构建pcDNA3.1-Rap2a质粒, 转染入A549和H1299细胞, 运用划痕和Transwell小室实验观察Rap2a对肺癌细胞侵袭能力的影响。CCK-8和流式细胞术检测Rap2a对细胞增殖和凋亡的影响, Western blot检测Bcl-2、Bax、ERK和Akt等的蛋白表达水平。
    结果 与空质粒组相比, 转染Rap2a质粒的肺癌细胞侵袭能力明显增加, 但细胞的增殖和凋亡能力无明显变化, Bcl-2和Bax的蛋白水平也无明显变化。Western blot结果显示, Rap2a过表达后p-Akt473的表达水平增加。
    结论 人Rap2a促进肺癌细胞的迁移侵袭可能与Rap2a影响Akt磷酸化水平有关。

     

    Abstract:
    Objective To detect the effect of Rap2a, a member of the small GTPase superfamily, on the invasion and metastasis of lung cancer cells and related mechanism.
    Methods The reconstructed plasmid pcDNA3.1-Rap2a was transfected into H1299 and A549 cells. The ability of invasion was evaluated by wound healing assay and Transwell test. The proliferation and apoptosis of the cells were measured by CCK-8 assay and flow cytometry. Meanwhile, the protein levels of Bcl-2, Bax, ERK and Akt were detected by Western blot.
    Results The ectopic expression of Rap2a promoted cancer cell invasion and migration, compared with the control group. However, no significant change in the proliferation and apoptosis of cancer cells were found in Rap2a-transfected cells, moreover, there was no detectable difference in Bcl-2 and Bax protein expression. Western blot analyses revealed that Rap2a overexpression significantly increased the phosphorylation level of AKT.
    Conclusion Rap2a promotes the cells migration and invasion, which might be via the regulation of AKT pathway in lung cancer cells.

     

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