Abstract: Objective To invesstigate the expression of FMNL-2 and MMP-2 in hepatocellular carcinoma (HCC) and the adjacent tissues, and to detect their relationship with the clinicopathological features. Methods The protein expression of FMNL-2 and MMP-2 were detected by immunohistochemistry MaxvisionTM method in the cancer tissues and adjacent tissues from 100 patients. Integrated optical density (IOD ) of immunohistochemical image was estimated by Image-ProPlus 6.0 software. Statistical analysis was performed by SPSS19.0 software. Results IOD of FMNL-2 between cancer tissues and adjacent tissues were (90 748.36±46 276.91)and (39075.46±24763.12);IOD of MMP-2 between cancer tissues and adjacent tissues were (87 969.46±27 131.77) and (59 517.72±20 218.23) (P<0.05); IOD of FMNL-2 between cirrhosis of liver tissue and non-cirrhosis liver tissue were(47 471.96±40 273.54) and (72 123.41±45 517.93), respectively(P<0.05); IOD of MMP-2 between HBsAg positive and negative group were (77 668.34±27 682.79) and (64 585.83±26173.62),respectively(P<0.05),IOD of MMP-2 between metastasis and without metastasis group were(63 854.73±21 730.42) and (77 218.05±28 930.01)(P<0.05),FMNL-2 expression was positively correlated to that of MMP-2 in cancer tissues and adjacent tissues (r=0.240,P=0.016). Conclusion FMNL-2 and MMP-2 proteins are abnormally expressed in hepatocellular carcinoma, indicating that detection of FMNL-2 and MMP-2 proteins may be helpful for diagnosis of hepatocellular carcinoma.