过表达外源FUT7对乳腺癌细胞黏附及迁移能力的影响

doi:10.3971/j.issn.1000-8578.2014.03.012

过表达外源FUT7对乳腺癌细胞黏附及迁移能力的影响

Effects of Overexpression of Exogenous FUT7 on Adhesion and Migration of Breast Cancer Cell

摘要

摘要: 目的观察过表达外源岩藻糖基转移酶VII（FUT7）对人乳腺癌MDA-MB-231细胞黏附和迁移能力的影响。方法构建以FUT7为靶基因的真核表达载体pEGFP-C1-FUT7，转染人乳腺癌MDAMB-231细胞，采用RT-PCR检测FUT7 mRNA的表达；Western blot检测FUT7及其催化产物sLeX蛋白水平；细胞黏附实验及Transwell小室检测转染后细胞与HUVECs细胞黏附、迁移能力。结果转染细胞外源FUT7表达在mRNA水平和蛋白水平及sLeX含量均明显增加；转染的乳腺癌细胞黏附和迁移能力明显增强。结论过表达外源FUT7基因可增强人乳腺癌MDA-MB-231细胞黏附和迁移能力。

Abstract: Objective To investigate the effect of exogenous overexpression of α1,3 FucT-Ⅶ(FUT7) on adhesion and migration of human breast cancer cell line MDA-MB-231. Methodes pEGFP-C1-FUT7 eukaryotic expression vector was constructed and transfected them into MDA-MB-231 cells. The expression level of FUT7 mRNA was examined by RT-PCR; Expression of FUT7 and catalysate sLeX protein were assayed by Western blot. Infl uence of exogenous FUT7 overexpression on adhesion and migration of MDAMB-231 cells to HUVECs was analyzed by adhesion assay and transwell assay. Results The exogenous expression levels of FUT7 mRNA，sLeX protein on cell surface were signifi cantly increased in transfected MDA-MB-231 which had enhanced abilities of adhesion and cell migration. Conclusion Overexpression of exogenous FUT7 promoted the abilities of adhesion and migration of human breast cancer cell line MDAMB-231.

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