miR-375和PDK1在胰腺癌组织中的表达及两者的相关性

Correlation between miR-375 and PDK1 Expression in Pancreatic Cancer Tissues

摘要: 目的探讨miR-375与PDK1在胰腺癌中的表达及两者的相关性。方法 qRT-PCR方法检测胰腺癌组织及胰腺癌细胞系中miR-375、PDK1表达，转染miR-375模拟物上调其在Panc-1中的表达，检测转染后Panc-1中PDK1表达变化。结果 miR-375在人胰腺癌组织中表达下调，在Panc-1中表达较HEK293明显降低。PDK1在胰腺癌组织中表达上调，在Panc-1中表达较HEK293明显增高。转染miR-375模拟物上调Panc-1中miR-375表达后，转染组PDK1表达较空白组和阴性对照组均明显下降。结论 miR-375在胰腺癌中发挥抑癌基因作用，对PDK1具有负调控作用。

Abstract: Abstract：Objective To investigate the expression of microRNA-375 (miR-375) and 3-phosphoinositide-dependent protein kinase-1 (PDK1) in pancreatic cancer and analyze their correlation. Methods miR-375 and PDK1 expression in pancreatic tissues and cell lines were evaluated using quantitative real-time PCR (qRT-PCR).After transfecting miR-375 mimics into Panc-1 cells,the expression of PDK1 mRNA and protein were assessed by qRT-PCR and Western blotting,respectively. Results Significant downregulation of miR-375 in pancreatic cancer and Panc-1 cells was identified,whereas PDK1 was upregulated in these samples.Transfection of miR-375 mimics led to downregulation of PDK1 in Panc-1 cells. Conclusion miR-375 may be a tumor suppressor in pancreatic cancer and regulate PDK1 negatively.