Abstract: Objective To observe the efficacy and adverse reaction of sequential chemotherapy consists of oxaliplatin,docetaxel and hydroxy camptothecine plus 5-fluorouracil to advanced gastric cancer(AGC). Methods Thirty-five patients were all treated with chemotherapy of L-OHP+5-Fu/CF，DOC+5-Fu/CF and HCPT+5-Fu/CF in sequence,every chemotherapy regimen was used continuously 2 cycles and repeated every 21 days.All patients received 6 cycles totally.Efficacy and toxicity were evaluated. Results The clinical efficacy has been evaluated in all 35 patients.The whole response rate（RR）was 60%（21/35）,the median time to progression (TTP) was 7.6 months，95% confidence interval(CI）ranged 7.0-8.2 months，the median overall survival（OS）was 15.1 months，95% CI ranged 14.2-16.0 months.The major toxic reactions included marrow suppression，nausea，vomiting，alopecia and allergic reactions，but all of them are reversible.No patients were dead from toxic reactions of chemotherapy. Conclusion The sequential chemotherapy of L-OHP,DOC and HCPT plus 5-Fu is an effective and tolerable regimen for AGC.The innovative chemotherapy schema deserves clinical expansion and further exploration in the future.