Abstract: Objective To investigate the expression of STAT-3 and Enolase-1 in breast cancer and its relationship with Estrogen receptor status,Progesterone receptor status,lymph node metastasis,clinical stages and histological grading. Methods The expression of STAT-3 and Enolase-1 were examined by immunohistochemical streptavid-peroxidase(S-P)assay in 126 cases of breast cancer and 26 cases of benign breast disease.And the significance of its expression was analyzed with clinicopathologic parameters. Results The expression rates of STAT-3(77.8％)and Enolase-1(82.5％)in breast cancer were significantly higher than that in benign breast disease（11.5％ vs.7.7％）and the expression of two proteins were positively correlated(χ²=42.416,P<0.05;χ²=57.211，P<0.05).The expression of STAT-3 and Enolase-1 in breast cancer has no significant difference in age,tumor size,histologic grading and clinical stages.Epression of STAT-3(85.7％)and Enolase-1(90.5％)in breast cancer with metastasis is significantly higher than that without metastasis （χ²=9.184,P<0.05;χ²=11.014,P<0.05）.There was a significant correlation between the high expression of STAT-3 and axillary lymphatic metastasis in comparisons between positive estrogen and/or progesterone receptor expression and Her-2 positive expression（P<0.05）.And there was a significant correlation between the high expression of Enolase-1 and axillary lymphatic metastasis in comparisons with positive estrogen or positive progesterone receptor expression and Her-2 positive expression（P<0.05）. Conclusion Both STAT-3 and Enolase-1 may be involved in the tumorgenesis development and metastasis of breast cancer.The high expression of STAT-3 and Enolase-1 in estrogen receptor or/and progesterone receptor or Her-2 positive breast cancer was associated with a high possibility of lymph node metastasis.The detection of STAT-3 and Enolase-1 may be helpful in the diagnosis and prognosis of breast cancer.