Abstract: Objective To study the effects of cellular immunity，IL-2 and TNF-α caused by Opioid drugs on hepatic cancer mice. Methods The models of H22 hepatic cancer mice were established by the cell inoculation method;and the animals were randomly divided into 5 groups.10 rats in each group were picked up randomly to detect of lymphocyte subsets CD3+,CD4+,CD8+and CD4+/CD8+ counts.The number of IL-2 and TNF-α were detected by Enzyme-linked Immunosorbent Assay(ELISA). Results Compared with the group Saline，the numbers of CD3+,CD4+,CD8+ and the ratios of CD4+/CD8+showed no significant different in group Pethidine（P>0.05），but decreased significantly in group Morphine，group Fentanyl and group Sufentanil（P<0.05）.Compared with group Saline，the numbers of IL-2 increased significantly in group Pethidine（P<0.05）;and the numbers of TNF-α showed no significant difference（P>0.05）;but the numbers of IL-2 and TNF-αdecreased significantly in group Morphine，group Fentanyl and group Sufentanil （P<0.05）. Conclusion Morphine,Fentanyl and Sufentanil can suppress the immune function，significantly decrease IL-2 and TNF-α of hepatic cancer in mice.Pethidine suppress the immune function unobviously，and the numbers of TNF-α has no significant difference，but significantly increase the numbers of IL-2.