Abstract: Objective To assess the relation between hyperthermia reduce glioma invasiveness and TNFR affinity. Methods Using immunohistochemical method and immunofluorescence method，respectively，to detect the expression and distribution of TNFR in tumor tissue.Radioligand binding assay of receptors method monitor the receptor binding rate of rhTNF after combination hyperthermia with rhTNF in C6 cells.Detection of C6 cells apoptosis by fluorescence spectrophotometric analyzed activity of Caspase3/7.Crystal violet staining method was used to detect the glioma invasiveness. Results In glioma tissue，TNFR2 expressed slightly on the tumor capillaries and tumor cells，TNFR1 expression mainly on the tumor cells.Hyperthermia 120 min group TNFR1 immunoreactivity had a significant increase.The receptor binding rate of rhTNF and activity of Caspase3/7 were significant increased and they both reached the peak at 120 min(compared with control group，P<0.01)，then decreased.At the same time，glioma invasiveness reached the minimum. Conclusion Hyperthermia increases TNFR1 expression and receptor binding rate of rhTNF further causes tumor cells apoptosis.This mechanism may explain the effect of hyperthermia on reducing glioma invasiveness.