扶正解毒颗粒对染镍大鼠肾细胞DNA单链断裂的影响

doi:10.3971/j.issn.1000-8578.2011.08.007

扶正解毒颗粒对染镍大鼠肾细胞DNA单链断裂的影响

Effects of Fuzheng Jiedu Granula on Nickel-induced DNA Damage in Rrats Renal Cells

摘要

摘要: 目的研究扶正解毒颗粒（FJG）对镍（NiSO4）诱发大鼠肾细胞DNA损伤的拮抗作用。方法以Wistar大鼠NiSO4 ［2.5 mg/(kg·d)］染毒造模，FJG灌胃处理，分别以单细胞凝胶电泳（SCGE）技术及比色法检测肾组织细胞DNA单链断裂及总抗氧化力（TAC）。结果与NiSO4组相比，FJG各组“彗星”样细胞出现率（计数一定量细胞中彗星样细胞所占的比例）、尾长、Olive尾矩、尾DNA%、尾长/头长比均明显降低（P<0.05和P<0.01），TAC则显著升高（P<0.05和P<0.01）。结论FJG对镍致细胞DNA损伤有一定的拮抗作用，其机制与抑制镍诱导氧化损伤有关。

Abstract: ObjectiveTo study the effect of Fuzheng Jiedu Granula（FJG）on DNA damage in rats renal cells induced by Nickel sulfate(NiSO4). Methods Rats nephrotoxicity was induced by intraperitoneal injection of NiSO4［2.5 mg/(kg·d)］ and different dosages of FJG ［5,10,20 mg/(kg·d)］were administered by gavage. Cellar DNA single strand break(SSB) and total antioxidant capacity (TAC) of renal tissues were determined with single cell gel-electrophoresis (SCGE) assay and colorimetry,respetively. ResultsThe percentage of comet-like tail cells,length of tail,tail Olive moment,tail DNA percentage and ratio of tail/head were increased as well as TAC was increased significantly in FJG groups, compered with those in NiSO4 group（P<0.05, P<0.01). ConclusionBy inhibiting oxidative damage,FJG could play in antagonistic effect on DNA damage induced by nickel.

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