皂角刺总黄酮诱导结肠癌HCT116细胞凋亡的作用
Apoptosis of Colon Cancer Cell Line HCT116 Induced by Flavone Components from Spina Gleditsiae
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摘要: 目的研究皂角刺总黄酮对结肠癌HCT116细胞凋亡的诱导作用。方法应用CCK-8试剂盒检测皂角刺总黄酮对细胞增殖的影响，Hoechst33258荧光染色、透射电镜观察细胞凋亡的形态学变化，流式细胞仪Annexin V/PI双染法检测细胞的凋亡率。结果皂角刺总黄酮能够显著抑制HCT116细胞的增殖，随着药物浓度的增加，抑制作用明显增强，IC50为(104.72±0.96) mg/L。皂角刺总黄酮作用48h后，形态学观察可见HCT116细胞核固缩、碎裂和凋亡小体等凋亡特征。流式AnnexinV/PI双染法检测显示:不同浓度的皂角刺总黄酮均可诱导HCT116细胞凋亡，在100 mg/L时细胞凋亡率为（35.61±3.76）%。结论皂角刺总黄酮能明显诱导结肠癌HCT116细胞凋亡，抑制其增殖。Abstract: ObjectiveTo study the apoptosis of HCT116 Colon cancer cell line induced by Flavone Components from Spina Gleditsiae. MethodsThe inhibition of cell proliferation was determined by CCK-8(Cell Counting Kit-8) assay. The morphological changes of the apoptosis cells were observed by optical electron microscope after Hoechst33258 fluorescent staining and transmission electron microscope. The apoptosis rate of HCT116 cells was observed by Flow Cytometry with Annexin-V/PI donble staining. ResultsFlavone Components from Spina Gleditsiae significantly inhibited the proliferation of HCT116 cells in a dose-dependent manner with IC50 of (104.72±0.96) mg /L. Colon cancer cells apoptosis was morphologically observed after were 48h induced by Flavone Components from Spina Gleditsiae. The apoptosis of HCT116 cells was induced by different of Flavone Components from Spina Gleditsiae and the apoptosis rate was（35.00±3.76）% at 100 mg/L. ConclusionFlavone Components from Spina Gleditsiae can induce the apoptosis of HCT116 cells and inhibit their proliferation.
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