Apolloon反义寡核苷酸对K562细胞增殖和凋亡的影响
Effects of Apollon Antisense Oligonucleotide on Proliferation and Apoptosis of K562 Cells
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摘要: 目的探讨Apollon反义寡核苷酸（antisense oligonucleotide，ASODN）对白血病细胞K562增殖及凋亡的影响。方法设计合成特异性的Apollon硫代磷酸ASODN及其对照错义寡核苷酸（missense oligonucleotide，MSODN），脂质体介导转染K562细胞后，采用MTT法检测K562细胞增殖，Annexin V-FITC法检测细胞的凋亡。结果Apollon反义寡核苷酸对K562细胞的生长抑制呈浓度-时间依赖性。Apollon ASODN在终浓度为600 nmol/L作用K562细胞时，能明显抑制其增殖，K562细胞凋亡率显著增加（P<0.01）。结论Apollon 反义寡核苷酸可下调Apollon基因表达，有效抑制K562细胞的增殖，并促进K562细胞凋亡。Abstract: ObjectiveTo investigate effects of Apollon antisense oligonucleotide (ASODN) on the proliferation and apoptosis of human leukemia(K562) cells. MethodsSpecific phosphorothioate ASODN and missense oligonucleotide(MSODN) of Apollon mRNA were synthesized and transfected into K562 cells following cationic liposome. The proliferation inhibition of K562 cells was assessed by MTT. The apoptosis rate was detected by Annexin V-FITC. ResultsApollon antisense oligonucleotide inhibited the cell proliferation in a dose and time dependent pattern. ASODN at a final concentration of 600nmol/L could significantly inhibit the K562 cell proliferation. The apoptosis rate was apparently increased (P<0.01). ConclusionApollon ASODN may decrease Apollon gene expression, suppress K562 cells proliferation effectively, and induce significant apoptosis of K562 cells.
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