靶向Bcl-xL基因siRNA在前列腺癌细胞增殖和凋亡中的作用

doi:10.3971/j.issn.1000-8578.2011.05.007

靶向Bcl-xL基因siRNA在前列腺癌细胞增殖和凋亡中的作用

Effect of siRNA Targeted against Bcl-xL on Cell Proliferation and Apoptosis of Prostate Cancer Cells

摘要

摘要: 目的研究Bcl-xL基因特异性RNA干扰对前列腺癌PC-3细胞株体外增殖能力和凋亡的影响。方法构建靶向Bcl-xL的小干扰RNA(siRNA)表达载体，转染PC-3细胞后，采用半定量RT-PCR和Western blot法检测转染前后Bcl-xL mRNA及蛋白表达水平的改变；采用噻唑兰(MTT)法检测细胞生长增殖情况；TUNEL试剂盒测定细胞的凋亡情况。结果靶向Bcl-xL的序列特异性的siRNA可以有效地抑制PC-3细胞Bcl-xL基因的表达。转染靶向Bcl-xL的siRNA表达质粒可以显著抑制PC-3细胞的增殖（P<0.001），诱导细胞凋亡（P<0.001）。结论Bcl-xL基因特异性RNA干扰可以显著抑制前列腺癌PC-3细胞的体外增殖并诱导细胞凋亡。

Abstract: ObjectiveTo investigate the effect of siRNA targeted against Bcl-xL on cell proliferation and apoptosis of PC-3 prostate cancer cells． MethodsA siRNA plasmid expression vector against Bcl-xL was constructed and transfected into PC-3 cells．The changes of Bcl-xL mRNA and protein expression were detected by semi-quantitive RT-PCR and Western blot respectively．Cell proliferation was detected by MTT assay．Cell apoptosis was evaluated by flow cytometry．ResultsThe sequence-specific siRNA effectively suppressed Bcl-xL expressing at both mRNA and protein levels．Bcl-xL expression significantly inhibited the proliferation(P<0.001)and induced apoptosis of PC-3 cells(P<0.001) respectively．ConclusionsiRNA targeted against Bcl-xL could effectively suppress PC-3 cell proliferation and significantly induce PC-3 cell apoptosis．

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