Abstract: ObjectiveTo investigate the possible association of TSP1 exon10 G1678A and exon13 A2210G polymorphisms with susceptibility to gastric cardia adenocarcinoma (GCA) in a population of North China. MethodsThe TSP1 G1678A and A2210G single nucleotide polymorphisms (SNPs) were genotyped by polymerase-chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis in 145 GCA patients and 198 healthy controls. ResultsThe overall genotype and allelotype distributions of TSP1 G1678A in GCA patients were not significantly different from those in healthy controls（P>0.05）. When stratified for smoking status and upper gastrointestinal cancers (UGIC) family history, compared with GG and GA genotype, AA genotype significantly increased the risk of developing GCA in non-smoker group ［age and gender and UGIC family history adjusted odds ratio (OR)= 1.79, 95%CI= 1.46~2.11］. The frequency of G allelotype was less than 1%, which indicated that TSP1 A2210G was not a common polymorphism in north China. ConclusionAA genotype of TSP1 G1678A may be one of the factors that affect the risk of developing GCA in non-smoking population in north China.