Abstract: Abstract:Objective To study mRNA expression levels of Ebp1 and their relations to clinical and pathological features in human esophageal cancer. Methods Ebp1 expression levels were quantified by real-time PCR assay from two hundred and twenty esophageal cancer tissues and from their matched adjacent normal esophageal tissues. Results One hundred and fifty（68.18%）samples were found up-regulation of Ebp1 in esophageal cancer comparing to the matched normal esophageal tissues. Univariate analysis showed that the Ebp1 mRNA expression levels were significantly associated with age, family history, lymph node metastasis, tumor size, differentiation, infiltrating depth and TNM stage. In contrast,the Ebp1 mRNA expression levels had to association with other clinical parameters (gender, pathological type and tumor position). Multiple factor analysis showed that family history, tumor size, differentiation, infiltrating depth and TNM stage were risk factors for Ebp1 however, pathological type was a protective factor as shown that Ebp1 mRNA expression levels in medullary type were higher than that in ulcerative type and fungating type. Conclusion Ebp1 plays an important role in the procession of esophageal cancer and might serve as a valuable target in monitoring of esophageal disease, metastatic potential and prognosis.