p42/44MAPK抑制剂U0126对人胃癌SGC-7901细胞增殖和凋亡的影响

Mechanism of Effect of p42/44MAPK Inhibitor U0126 on SGC-7901 Cell Proliferation and Apoptosis

摘要: 目的研究U0126对人胃癌SGC-7901细胞生物学特性的影响。方法用MTT法测定U0126对SGC-7901细胞增殖的影响，流式细胞仪检测U0126对SGC-7901细胞周期和细胞凋亡的影响，用Western blot分析p42/44和p38以及其磷酸化水平的变化。结果 10、15、20μM U0126均能够抑制胃癌SGC-7901细胞的增殖；可使S和G2/M期肿瘤细胞比例减少，G₀/G₁期肿瘤细胞比例增加，其中20μM浓度的U0126作用最强(P<0.01)；U0126诱导肿瘤细胞凋亡，其中20μM浓度的U0126凋亡作用最强(P<0.01)；U0126使p-p42/44下调而使p-p38上调。结论 U0126诱导肿瘤细胞凋亡，抑制肿瘤细胞的增殖，其机制可能是抑制p-p42/44表达，并使p-p38表达增强。

Abstract: Objective To study the effect of U0126 on cytobiological characteristics of SGC-7901. Methods To determine effect of U0126 on SGC-7901 cell proliferation by MTT.Flow cytometry was used to detect influence of U0126 on SGC-7901 cell cycle and apoptosis.The change of p42/44,p38,p-p42/44 and p-p38 was analyzed by Western blot. Results 10，15，20μM U0126 can restrain SGC-7901 cell proliferation，decrease S and G₂/M phase cell ratio and increase G₀/G₁phase cell ratio.U0126 can induce tumor cell apoptosis and have a most powerful apoptosis in 20μM(P<0.01).U0126 can decrease p-p42/44 and increase p-p38. Conclusion U0126 can induce tumor cell apoptosis and inhibit cell proliferation.