MFAP3L在胃癌中的表达及与PI3K/AKT信号转导通路的关系

doi:10.3971/j.issn.1000-8578.2010.07.019

MFAP3L在胃癌中的表达及与PI3K/AKT信号转导通路的关系

MFAP3L Expression in Gastric Cancer and Potential Role in PI3K/AKT Pathway

摘要

摘要: 目的：了解微纤维关联样蛋白-3（MFAP3L）基因在胃癌组织中的表达情况并分析其与PI3K/AKT信号转导通路相关基因间的关系。方法:采用半定量RT-PCR和组织微阵列免疫组化的方法分别检测MFAP3L、磷脂酰肌醇-3-激酶催化亚基α（PIK3CA）、蛋白激酶B-β（AKT2）及β-半乳糖凝集素-3（Glaectin-3）基因在胃癌组织中mRNA及蛋白表达情况，并通过生物信息学方法预测分析MFAP3L蛋白结构特点。结果:（1）在同一组胃癌组织中，MFAP3L、Glaectin-3及PIK3CA蛋白均表达上调（17/24，70.8%，P=0.022；16/25，64.0%，P=0.017及14/25，56.0%，P=0.045）；PIK3CA与AKT2基因mRNA均表达上调（19/29，65.5％，P=0.001与14/25，56％，P=0.001）。并且MFAP3L与Glaectin-3及PIK3CA间存在共表达。(2) MFAP3L蛋白含有多个磷酸化位点, 且在胞内区聚集成簇，并具有1个与PI3K的SH3调节区高度同源的蛋白模序。结论: MFAP3L基因在胃癌组织中的表达变化可能与胃癌的发生有密切关系，并可能通过参与PI3K/AKT 通路来参与肿瘤侵袭和转移。

Abstract: Objective：To investigate the MFAP3L expression in gastric cancer (GC) and its potential role in the PI3K/Akt pathway. Methods ：RT-PCR and microarray IHC were performed to detect the mRNA and protein expression of MFAP3L, PIK3CA, AKT2 and Glaectin-3 in primary stomach tumors.Bioinformatics Methods： were used to analyze the protein structure of MFAP3L. Results ：MFAP3L,Glaectin-3 and PIK3CA protein were highly expressed in primary stomach tumor (70.8%, 17/24, P=0.022; 16/25, 64.0%, P=0.017; 14/25, 56.0%, P=0.045).PIK3CA gene mRNA was highly expressed in GC (65.5% 19/29, P=0.001) as well as AKT2 mRNA (56.0%, 14/25, P=0.001).Glaectin-3 protein co-expressed with Glaectin-3 and PIK3CA protein.Additionally, MFAP3L contains multiple phosphorylation sites and a conservative transmembrane domain. Conclusion ：This study demonstrated that high expression of MFAP3L may be relevant to stomach tumorigenesis and get involved with PI3K/Akt pathway to facilitate tumor invasion and metastasis.

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