Abstract: Objective：To investigate the role of CD151 and intergrin α3β1 proteins in the occurrence development and invasion and metastasis of breast cancer. Methods ：Tissue microarray (TMA) which contained 40 cases of normal tissues and 130 cases of various kind of breast cancer was manufactured.Protein levels of CD151 and intergrinα3β1 were detected by using immunobiochemical steptavidin peroxidase method (SP).The χ² test and Spearman analysis were used.Statistical significance was defined as a P value< 0.05. Results ：The positive rates of CD151 and integrinα 3β1 expressions were 0.0%（0/39）and 7.7%(3/39) in normal breast tissues; 33.3%（10/30）and 30%(9/30) in tissues of carcinoma in situ, respectively; 59.0%(59/100) and 70.0%（70/100）in cancerous tissues, respectively.The differences between two kinds of protein expression rates among the three groups were significant (χ² =41.28, χ²=48.62).The expressions of CD151 and intergrinα3β1 protein in breast cancers have no relationship with patient's age the histological type and size of cancer but significantly related with differentiated degree of tumor lymph nodes metastasis and clinical stage.(P<0.05）.In particular, it is worth noting that there is significantly relation between the expression of CD151and ER/ PR (P<0.05).There is positive correlation between the expression of CD151 and intergrinα3β1 protein(r=0.565; P<0.01). Conclusion ：The CD151 and intergrinα3β1 were over expressed in breast carcinoma, which suggested that two proteins were related to oncogenesis, development, invasion and metastasis of breast carcinoma.Joint detection of the two makers can be used to determine the biological behavior and prognosis of breast cancer.