RNAi下调hTER长T基因对MCF-7乳腺癌细胞生的影响

doi:10.3971/j.issn.1000-8578.2010.07.008

RNAi下调hTER长T基因对MCF-7乳腺癌细胞生的影响

Effect of Targeting hTERT Gene Inhibited in MCF-7 Breast Cancer Cell Growth by RNA Interference

摘要

摘要: 目的：利用siRNA在乳腺癌细胞内诱导RNAi，抑制人端粒酶催化亚基（human telomerase reverse transcriptase，hTERT）基因表达，在体外细胞水平探讨RNAi对MCF-7乳腺癌细胞生长的影响。方法：构建靶向hTERT基因的siRNA（hTERT-siRNA），转导入MCF-7乳腺癌细胞，在瘤细胞内诱导RNAi，采用细胞增殖抑制实验、RT-PCR法、Western blot等技术检测siRNA处理前后瘤细胞增殖及hTERT基因表达变化。结果：hTERT-siRNA对乳腺癌细胞生长抑制率达61.94%；hTERT的mRNA表达降低了61.7%，蛋白表达平均降低了52.8%。结论：siRNA在体外明显抑制了乳腺癌细胞中hTERT基因的表达和瘤细胞增殖。

Abstract: Objective：To investigate the effect of breast cancer cell growth, we utilized RNAi tragedy to inhibit hTERT gene in MCF-7 cells of breast cancer. Methods：Small intererence RNA (siRNA) homologous to hTERT gene (hTERT-siRNA) was recombined with pGenesil-1 vector, then transfected into MCF-7 cells of breast cancer to induce RNAi,and then hTERT gene expression alterations and tumor cell proliferation in both hTERT-siRNA treatment groups and control groups were detected by MTT assay, RT-PCR and Western blot. Results：The expression of hTERT had been obviously inhibited by siRNA.The inhibition rate of cell proliferation was 61.94%.The mRNA level of hTERT was obviously decreased by 61.7%, and its protein expression reduced by 52.8%. Conclusion：siRNA-hTERT effectively inhibited hTERT gene expression and breast cancer cell proliferation in vitro.

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