TGF-β1 shRNA对涎腺人黏液表皮样癌裸鼠颌下腺移植瘤的抑瘤作用及Ki-67、VEGF蛋白表达的影响

doi:10.3971/j.issn.1000-8578.2010.07.001

TGF-β1 shRNA对涎腺人黏液表皮样癌裸鼠颌下腺移植瘤的抑瘤作用及Ki-67、VEGF蛋白表达的影响

Inhibitory Effect of TGF-β1 shRNA on Human Salivary Gland Mucoepidermoid Carcinoma in Nude Mice Submandibular Gland and Effect of Ki-67 and VEGF Expression

摘要

摘要: 目的研究TGF-β1 shRNA对人涎腺黏液表皮样癌（Ms）细胞裸鼠颌下腺原位移植瘤成瘤能力以及对Ki-67和VEGF蛋白表达的影响。方法将Ms细胞、稳定转染空载体的Ms细胞、稳定转染TGF-β1 shRNA的Ms细胞分别接种至裸鼠颌下腺，观察各组细胞在裸鼠体内的成瘤率，测量肿瘤体积、瘤质量并绘制肿瘤生长曲线；免疫荧光法和免疫组织化学法检测肿瘤组织中Ki-67和VEGF蛋白的表达。结果与未转染组、转染空载体组相比，转染TGF-β1 shRNA组裸鼠颌下腺原位移植瘤生长缓慢，瘤体体积和瘤质量显著降低(P< 0.01)，瘤细胞中Ki-67和VEGF蛋白表达明显降低。结论 TGF-β1 shRNA能显著抑制Ms细胞在体内的生长能力，其机制可能与下调细胞内Ki-67和VEGF水平有关。

Abstract: Objective To study inhibitory effect of TGF-β1 shRNA on human salivary gland mucoepidermoid carcinoma in nude mice submandibular gland and the effect of Ki-67 and VEGF expression. Methods Nude mice submandibular gland were transplanted with Ms cells, Ms cells stably transfected with empty vectors, Ms cells stably transfected with TGF-β1 shRNA, respectively.The tumor volume and weight were measured and observed by growth curve.Immunohistochemistry and immunoflurescence were used to detect the level of Ki-67 and VEGF. Results Compared with Ms cells and Ms transfected with empty vectors, Ms cells stably transfected with TGF-β1 shRNA showed slow growth in tumor volume and weight (P< 0.01), as well as down-regulated the expressions of Ki-67 and VEGF. Conclusion TGF-β1 shRNA could inhibit the tumor growth of Ms cells, the mechanism of which might be related with down-regulation of Ki-67 and VEGF.

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