Abstract: Objective To study the significance of FOXO1A, FLASH expression and analyzed their relationship with tumor clinicopathological features. Methods FOXO1A and FLASH expression were examined by immunohistochemical staining method in 68 cases of human gastric cancer compared with human normal gastric tissue. Further analysis was performed to make sure their relationship with some tumor clinicopathological features. Results In gastric carcinomas, the expression of FOXO1A, FLASH were 73.53%(50/68) and 77.94%(53/68) respectively. There were no significant associations between FLASH and age, tumor location, tumor size, depth of penetration or lymphatic metastasis. Besides grading, the expression of FOXO1A was related to gender too and higher in male. There was no significant correlation between the expression of FOXO1A and FLASH. In intraepithelial neoplasia gastric mucosa, the expression of FOXO1A and FLASH presents intensive positively. Their expression in epithelial cells of normal gastric mucosa was shown as no or very weak intensity. Conclusion In conclusion, our results showed that the expression of FOXO1A and FLASH is a frequent event in highly differentiated gastric carcinoma, and may play important role in tumorigenesis and tumor development. There was no significant correlation between the expression of FOXO1A and FLASH.