Abstract: Objective：To investigate the expression of excision repair cross complementing-1(ERCC1)，P27^kip1 and CyclinE in gastric cancer and explore its role in development of gastric carcinoma and its clinical significance. Methods：Expression levels of ERCC1,P27^kip1 and CyclinE in 45 resected patients with gastric cancer and 10 normal tissues were detected by the method of immunohistochemistry（EliVisionTM）. We analyzed its correlation withgastric cancer in χ² test and pearson analyze. Results：The positive rates of ERCC1，P27^kip1 and CyclinE protein expression was 31.1%，35.6% and 55.6% in gastric cancer，respectively. The positive rate of ERCC1 and P27^kip1 in gastric cancer was lower than that in comparison group（ P <0.05）；The expression of CyclinE showed a significant difference from those of normal gastric mucosa ( P <0.05). The expression level ofERCC1，P27^kip1 and CyclinE was related to serosa invasion,lymphnode metastasis,distant metastasis and clinical histological grade( P <0.05). However, the expression of ERCC1,P27^kip1 and CyclinE was not associated with gender，age，tumor size，position and differentiation. There was a highly negative correlation between P27^kip1 and CyclinE expression ( P <0.05). Conclusion：The high expression of ERCC1,P27^kip1 and the low expression of CyclinE were related to occurrence and development of gastric cancer. The detection of ERCC1，P27^kip1 and CyclinE may be used for the diagnosis,identification and prognosis in patients with gastric cancer. There was anegative correlation between P27^kip1 and CyclinE protein expression, but there was not correlation between expression level of ERCC1,P27^kip1 and CyclinE in gastric cancer.