酵母双杂交筛选原癌基因proto-Dbl的上游调控分子

Screening of Upstream Regulators of Proto-Dbl Oncoprotein by Yeast Two-hybrid System

摘要: 目的：探讨VL domain对Dbl癌基因活性的调控机制。方法：本文通过酵母双杂交的方法，以proto-Dbl的VL domain为诱饵蛋白对人胎脑文库进行筛选。结果：研究共获得7个能与VL domain相互作用的蛋白。结论：Proto-Dbl VL domain相互作用蛋白的发现将为揭示proto-Dbl的功能奠定基础。

Abstract: Objective：The VL domain truncate mutation could directly cause the activation of proto-Dbl and the malignant transformation. Methods：In order to clarify the activation mechanism of proto-Dbl, yeast two-hybrid system was used to screen the proteins interacted with the VL domain in the human fetal brain cDNA library taking VL domain as the bait. Results：7 positive clones which interact with proto-Dbl-VL domain were successfully harvested. Conclusion：This discovery lays the foundation for uncovering of the regulatory mechanism of proto-Dbl.