槲皮素对人乳腺癌细胞MDA-MB-435S增殖及侵袭力的影响

doi:10.3971/j.issn.1000-8578.2009.07.003

槲皮素对人乳腺癌细胞MDA-MB-435S增殖及侵袭力的影响

Effect of Quercatin on Proliferation and Invasion of Human Breast Carcinoma Cell Line MDA-MB-435S

摘要

摘要: 目的探讨槲皮素对人乳腺癌细胞MDA-MB-435S 增殖及侵袭能力的影响。方法人乳腺癌细胞MDA-MB-435S以12.5、25、50、100、200μM 终浓度的槲皮素处理后，台盼蓝拒染法检测细胞的增殖状况、流式细胞术法检测细胞的凋亡率、Boyden小室法检测细胞的侵袭能力。结果人乳腺癌细胞MDA-M-435S经槲皮素处理后，其体外增殖及侵袭能力明显下降的同时凋亡率明显上升，且与药物的剂量呈正相关，当槲皮素终浓度达到200μM时，MDA-MB-435S细胞的生长及侵袭能力几乎完全受到抑制。结论槲皮素在体外剂量依赖性的抑制人乳腺癌细胞MDA-MB-435S的增殖及侵袭能力并能诱导其凋亡，为槲皮素用于乳腺癌的预防和治疗提供了部分依据。

Abstract: Objective To investigate the effect of quercetin on the proliferation and invasion in human breast cancer cells. Methods The proliferate capacity of human breast carcinoma cell line MDA-MB-435S was measured using tryan blue dye exclusion test, the apoptosis rate was analyzed using flow cytometry and the invasive capacity was measured using Boyden chamber invasive model after the cells were treated with different concentrations of quercetin (12.5,25,50,100 and 200μM) . Results Quercatin could inhibit the proliferation and invasion and induce apoptosis of MDA-MB-435S cells at dose dependent relationships. With 200μM of quercatin the growth and invasion of MDA-MB-435S cells were almost completely inhibited. Conclusion Quercatin could inhabit the proliferation and invasion and induce apoptosis of human breast carcinoma cell line MDA-MB-435S at dose dependent patterns, which could provide some bases for the clinical application of quercatin for breast cancer therapy in the near future．

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