Abstract: Objective To explore the roles and the significances of angiopoietin-2（Ang-2),vascular endothelial growth factor（VEGF）and VEGFR-3 expression and their relationship with clinicopathologic characteristics of larynx squamous cell carcinoma. Methods Immunohistochemical staining was used to detect Ang-2,VEGF and VEGFR-3 expression in larynx squamous cell carcinoma and adjacent normal tissue. Results The expression level of Ang-2,VEGF and VEGFR-3 was significantly higher in larynx squamous cell carcinoma than in adjacent normal tissue（Ang-2,P<0.01;VEGF,P<0.01;VEGFR-3,P<0.01). There was no significant correlation between Ang-2 expression and tumor differentiation or lymph node metastasis（P>0.05). VEGF and VEGFR-3 expression was correlated with lymph node metastasis（P<0.05) but not tumor differentiation（P>0.05). Positive correlation was found between expression of Ang-2 and VEGF（r=0.8193，P<0.01), also between expression of VEGF and VEGFR-3（r=0.7365，P<0.01). Conclusion Expression of Ang-2,VEGF and VEGFR-3 was closely associated with tumor angiogenesis and maturity.Their overexpression may play a major role during the development of larynx squamous cell carcinoma.