Abstract: Objective To investigate the inhibitory effect of EFE on the growth of xenografted tumor of hepatocellular carcinoma (HCC) and the expression of CD44v6. Methods First, tumor bearing models xenografted with SMMC-7721 cells were developed in nude mice, then the mice were divided randomly into saline group, EFE group, 5-Fu group and combined group. Calculating the inhibition ratio of tumor growth and coefficient of drug in interaction. Evaluating the adverse reaction of EFE by blood routine tests. At the same time, the expression of CD44v6 was detected by SP staining of immunohistochemistry, RT-PCR and Western blot in EFE and saline groups. Results Compared with saline group, inhibition ratios of tumor growth in EFE group and 5-Fu group are 20.53%，23.07%respectively. The inhibition ratio of combined group is 54.20%, and CDI<1. Blood routine tests show that EFE has no significant adverse reaction to hematopoietic system. EFE could obviously inhibit the expression of CD44v6 protein by immunohistochemistry and Western blot, the inhibitory rates were 47.16% and 28.37% respectively(P<0.05）. And the result of RT-PCR also showed that EFE could obviously inhibit the expression of CD44v6 mRNA, the inhibitory rate was 16.44% (P<0.05）. Conclusion EFE has inhibitory effect on hepatoma xenografted tumor to some extent and has synergistic anti-tumor activity with 5-Fu. Moreover, EFE could obviously inhibit the expression of CD44v6.