皂角刺提取物对荷瘤小鼠肿瘤生长及细胞因子的影响

doi:10.3971/j.issn.1000-8578.2009.05.002

皂角刺提取物对荷瘤小鼠肿瘤生长及细胞因子的影响

Effects of Extractive from Spina Gleditsiae (ESG)on Tumor Growth and Cytokines in Tumor-bearing Mice

摘要

摘要: 目的探讨皂角刺提取物对荷瘤小鼠肿瘤生长及细胞因子水平的影响。方法建立小鼠肉瘤S180及小鼠肝癌H22移植性肿瘤模型，观察皂角刺提取物对荷瘤小鼠肿瘤生长的抑制作用；采用ELISA法测定白介素-2(IL-2)、白介素-6(IL-6)、白介素-12(IL-12)、肿瘤坏死因子-α(TNF-α)的水平，探讨皂角刺提取物对荷瘤小鼠细胞因子的影响。结果皂角刺提取物在50、100 mg/kg时对S180的生长抑制率分别为44.59%、53.38%，对H22生长抑制率分别为39.88%、46.63%，且能提高荷瘤小鼠的脾指数和胸腺指数；皂角刺提取物能提高荷瘤小鼠细胞因子IL-2、IL-6、IL-12、TNF-α的表达水平。结论皂角刺提取物有较强的体内抗肿瘤活性，且能促进细胞因子的表达，增强机体免疫功能。

Abstract: ObjectiveTo study the effects of Extractive from Spina Gleditsiae (ESG)on the tumor growth and cytokines in tumor-bearing mice. MethodsAntitumor activity of ESG in vivo was observed by the models of implanted H22 or S180 cell line in mice. The expression of TNF-α、IL-2、IL-6 and IL-12 in tumor-bearing mice was detected by ELISA assay. Results At dosage of 50、100mg•kg-1,inhibitory rate of ESG is 44.59% and 53.38% for S180, 39.88% and 46.63% for H22, respectively. ESG can increase the expression of TNF-α、IL-2、IL-6 and IL-12. Conclusion ESG exhibits anti-tumor effect obviously in vivo and enhances immune function by promoting the expression of cytokine.

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