探讨bcl-2下调后对胶质瘤细胞系的增殖、凋亡和侵袭能力的影响

doi:10.3971/j.issn.1000-8578.2009.02.004

探讨bcl-2下调后对胶质瘤细胞系的增殖、凋亡和侵袭能力的影响

Effect of Bcl-2 Downregulation on Proliferation, Apoptosis and Invasion of Glioma Cell Lines

摘要

摘要: 目的研究下调bcl-2基因表达后对神经胶质瘤细胞增殖、凋亡和侵袭能力的影响。方法利用小分子RNA干扰（Small interfering RNA，siRNA）技术人工合成bcl-2靶向siRNA，转染神经胶质瘤细胞系SHG44和TJ905细胞，特异性沉默神经胶质瘤细胞SHG44和TJ905细胞bcl-2基因表达。通过蛋白质印迹（Western blot）分析、噻唑蓝（MTT）实验、Transwell实验和流式细胞仪（FCM）检测，观察bcl-2 siRNA对转染细胞bcl-2基因抑制效果、增殖能力的变化、侵袭能力的改变和细胞凋亡的影响。结果 bcl-2 siRNA可特异性下调神经胶质瘤细胞系SHG44和TJ905细胞bcl-2基因表达。bcl-2下调的神经胶质瘤细胞增殖能力在不同时间点都受到抑制，侵袭能力也明显下降，但实验中未见细胞明显凋亡。结论下调bcl-2基因表达可有效抑制神经胶质瘤细胞生长，降低细胞增殖和侵袭能力。

Abstract: Objective To study how bcl-2 downregulation influence on proliferation, apoptosis, and invasion of glioma cell lines. Methods bcl-2 siRNA was chemically synthesized and transfected into glioma cell line SHG44 and TJ905. The silencing of bcl-2 expression in two cell lines were checked by Western blot.. The effect of bcl-2 supression on proliferation, invasion, and apoptosis of two cells were evaluated via MTT, cell transwell assay and Flow cytometric method. Results bcl-2 siRNA were effectively inhibited the expression of bcl-2 gene in SHG44 and TJ905 glioma cell lines. Silencing bcl-2 gene expression leads to the growth suppression of both SHG44 and TJ905 cells at different time point.Similarly, two cells invasion abilities were significantly reduced. However, no apparent effect on apoptosis was observed. Conclusion Our results have indicated that bcl-2 gene silencing effectively inhibited growth, proliferation and invasion of glioma cells.

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