子宫内膜癌VEGF2D、MLD 的表达及其与 淋巴结转移的关系
Relationship between Expression of VEGF2D and MLD and Lymph Node Metastasis in En2 dometrial Carcinoma
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摘要: 目的 探讨子宫内膜癌组织中血管内皮生长因子D (VEGF2D) 和微淋巴管密度(MLD) 的表达与淋巴结转移的关系。方法 选取子宫内膜癌标本53 例,正常子宫内膜标本20 例,以淋巴管特异性标记物D2240 标记淋巴管并计数MLD, 运用免疫组织化学技术检测VEGF2D 和MLD 在子宫内膜癌、正常内膜组织中的表达。结果 (1) VEGF2D 和MLD 在子宫内膜癌组织中的阳性表达率及密度值(39/53, 23. 54 ±7. 84) 均显著高于正常内膜组织(5/ 20, 10. 48 ±1. 62) ( P < 0. 05) ;两者在淋巴结转移组的阳性表达率及密度值(33/ 38, 32. 05 ±3. 27) 也明显高于无淋巴结转移组(6/ 15, 20. 23 ±4. 57) ( P < 0. 05) ;VEGF2D 的表达在不同年龄、不同组织分化程度、不同浸润深度之间差异均无统计学意义( P > 0. 05), 在临床分期的表达中差异有统计学意义( P < 0. 05) 。(2) 子宫内膜癌VEGF2D 的表达与MLD 存在相关性,相关系数rs = 0. 398 ( P < 0. 05), VEGF2D 阳性组MLD (31. 17 ±2. 82) 较VEGF2D 阴性组MLD (16. 75 ±3. 63) 显著增高( P < 0. 05) 。结论 VEGF2D 在子宫内膜癌中呈高表达且与微淋巴管密度(MLD) 呈正相关,其可能通过诱导淋巴管生成促进子宫内膜癌淋巴结转移,可作为指导子宫内膜癌诊治及判断预后的一个重要指标。Abstract: Objective To analyze the relationship between expression of vascular endothelial growth factor D (VEGF2D) and the microlymphatic density (MLD) and lymph node metastasis in endomet rial carcino2 ma. Methods Immunohistochemical method was used to detect VEGF2D and MLD in 53 cases of endom2 et rial carcinoma and 20 cases of normal endomet ria. Lymphatic vessels were marked by D2240 and the mi2 crolymphatic density (MLD) was counted by Masakazu's method. Results (1) The positive expression rate of VEGF2D and MLD in the endomet rial carcinoma tissue (39/ 53, 23. 54 ±7. 84) were much higher than that in normal endomet ria tissue (5/ 20, 10. 48 ±1. 62) ( P < 0. 05) ; the positive expression rate of VEGF2D and MLD in lymph2node2positive group (33/ 38, 32. 05 ±3. 27) were much higher than that in lymph2node2negative group (6/ 15, 20. 23 ±4. 57) ( P < 0. 05) ; the positive expression rate of VEGF2D in endomet rial carcinoma had no correlation with age, differentiation and depth of invasion ( P > 0. 05 re2 spectively), but was significantly related to clinical stage ( P < 0. 05) . (2) The positive expression of VEGF2D had correlation with MLD ( rs = 0. 398, P < 0. 05 ) . The MLD of the positive group of the VEGF2D (31. 17 ±2. 82) was higher than in the negative group (16. 75 ±3. 63) ( P < 0. 05) . Conclusion The positive expression of VEGF2D in endomet rial carcinoma has significantly correlation with MLD and it may induce lymphangiogenesis and promote the lymph node metastasis of endomet rial carcinoma. The VEGF2D may be used as a valuable index in evaluating diagnosis, t reatment and prognosis of endomet rial carcinoma.
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