放射性骨损伤愈合能力降低机制的实验研究

Experimental study on the mechanism of decrease in bone healing capacity after irradiation

摘要: 目的　探讨放射性骨损伤 (RBI)愈合能力降低的机制。方法　6 0 Coγ射线 15Gy单次照射并致兔右胫骨骨折, 以半环槽氏外固定器固定, 未照射组为对照组。术后 3d、1、3、6、12、2 4周通过组织学、血流量测定及免疫组化 (BMP与TGF β1)观察骨折愈合情况。结果　实验组骨折愈合较对照组晚 12～ 16周, 且骨折端血流量明显降低 ;BMP与TGF β1的表达亦低于对照组。结论　RBI愈合能力下降的原因是骨组织血运障碍与抑制BMP与TGF - β1的综合作用。

Abstract: Objective To investigate the mechanism of decrease in bone healing capacity after irradiation. Methods After the right hind shank of rabbits was irradiated with 60 Co γ ray at a dose of 15Gy,. the transverse fracture of the upper-middle tibia was inflicted with electric saw. Then, the Half-ring External Fixator was placed. Unirradiation group was called the control group. The therapeutic effects were observed with histological examination, BMP-McAb and TGF-β1-McAb immunohistochemistry, measuremen...