大肠癌细胞间缝隙连接通讯与E-钙粘素的检测

Detect of GJIC and E-Cad on colorectal cancer

摘要: 目的　定性检测大肠癌组织缝隙连接蛋白 (Cx4 3)与E 钙粘素 (E Cad)的表达 ;定量测定正常上皮与大肠癌细胞间缝隙连接通讯 (GJIC)。方法　鼠大肠癌与对照组织中Cx4 3和E Cad的表达用免疫组化法检测 ;GJIC用荧光漂白恢复法检测。结果　大肠癌Cx4 3和E Cad阳性率 (40 %, 4 3% )明显低于对照组织(90 %, 87% ), 33%肿瘤标本二者皆不表达, 该组肿瘤的分化度也较差 ;大肠癌细胞HR, Lovo, HT 2 9间GJIC明显低于正常上皮细胞。结论　在鼠大肠上皮细胞恶变过程中伴有Cx4 3与E Cad的表达抑制或缺失与大肠癌的发生发展有关。

Abstract: Objective Detect the expression of Cx43 and E-Cad on rat colorectal cancer tissues and normal control colon tissues.Quantitative determination GJIC in colorectal cancer cells and normal control cells. Methods Detect Cx43 and E-Cad on rat colorectal cancer tissues and normal colon tissues with immunohistochemistry method,test GJIC level on three colorectal cancer cells and one normal control cell by fluorescence photobleaching recovery method. Results The positive rate of Cx43 and E-Cad (40%?43%) on colo...