慢性髓性白血病来源的树突状细胞诱导的CTL体外作用研究

The in Vitro Response of Cytotoxic T Lymphocyte Induced by Chronic Myeloid Leukemia Derived Dendritic Cells

摘要: 目的　研究慢性髓性白血病来源的树突状细胞的免疫功能。方法　分离慢性期CML患者外周血单个核细胞 (PBMNC), 用细胞因子GM CSF及IL 4在体外诱导培养DC, 检测细胞表型, 并观察其诱导的CTL体外抗肿瘤效应。用酶联免疫 (ELISA)方法测定CML DC混合淋巴细胞反应 (MLR)上清液中IL 12及IFN γ的量, 并与正常DC进行比较。结果　联合GM CSF及IL 4可诱导CML细胞分化为CML DC, CML DC的CD1a、CD80、CD83表达率均低于正常DC ;CML DC混合淋巴细胞反应上清IL 12及IFN γ含量均低于正常DC ;CML DC能诱导出对自身CML细胞有特异性杀伤作用的CTL。结论　CML DC具有抗原提呈细胞的免疫功能, 能诱导抗白血病CTL反应。

Abstract: Objective To investigate the immune function of dendritic cells derived from chronic myeloid leukemia(CML-DC). Methods The mononuclear cells were separated from the peripheral blood of CML patients and cultured with GM-CSF and IL-4 to induce DC. The phenotype of CML-DC and the anti-tumor effects of cytotoxic T cells induced by CML-DC were investigated. The level of IL-12 and IFN-γ in supernate were measured by ELISA. CML-DCs were compared with normal DCs. Results It was proved that the DCs could be der...