B-ALL病人TCR Vβ基因谱系和克隆性分析

Analysis of T-cell Receptor Vβ Gene Repertoire and Clonality in Patients With B-ALL

摘要: 目的　了解B ALL病人外周血T细胞的TCRVβ基因谱系及其克隆性增殖情况。方法　利用RT PCR方法扩增 13例初发未治B ALL病人外周血单个核细胞中 2 4个TCRVβ基因的互补决定区 3(CDR3), PCR产物进一步经荧光标记和基因扫描分析CDR3长度而确定T细胞的克隆性。结果　 13例B ALL病人分别表达 2～ 18个Vβ亚家族。 13例病人均存在 1个或多个Vβ亚家族的克隆增殖T细胞, 增殖形式包括寡克隆及寡克隆增殖趋势、双克隆和单克隆。Vβ2 1和Vβ2 3亚家族出现克隆增殖性T细胞的频率较高。结论　B ALL病人外周血T细胞的TCRVβ谱系呈现优势利用的特点, 并存在克隆性增殖的T细胞, 这可能是机体对白血病相关抗原产生的特异性免疫反应 ;Vβ2 1和Vβ2 3亚家族T细胞发生克隆增殖改变的趋向性比较明显, 可能与B ALL相关抗原刺激有关

Abstract: Objective To investigate the situation of TCR Vβ gene repertoire and clonal expansion in peripheral blood T cells from patients with B ALL. Methods The complementarity determining region 3 (CDR3) of TCR Vβ 24 subfamily genes in peripheral blood mononuclear cell from 13 cases with untreated B ALL were amplified using RT PCR. The PCR products were further labelled by fluorescein and then analyzed by genescan technique for detection of the CDR3 size, to determine clonality of T cells. Results 2～18 Vβ subfa...