血管内皮生长因子受体-3对宫颈癌细胞凋亡的效应研究

The Effect of Vascular Endothelial Growth Factor Receptor-3 on the Apoptosis of Cervical Cancer

摘要: 目的研究血管内皮生长因子受体-3(VEGFR-3)在人宫颈癌细胞凋亡过程中的作用。方法用基因重组方法构建人反义VEGFR-3基因真核表达载体,用电穿孔法转染人宫颈癌细胞系Hela细胞。采用WesternBlot分析转染前后细胞中VEGFR-3蛋白的变化。利用Hoechst33258染色和流式细胞仪观察转染后Hela细胞的凋亡情况。结果转染反义VEGFR-3质粒后,Hela细胞VEGFR-3的蛋白表达水平明显下降,细胞凋亡率明显增加(P<0.01)。结论抑制VEGFR-3的表达可促进宫颈癌细胞的凋亡,VEGFR-3可能是肿瘤基因治疗的一个潜在新靶点。

Abstract: Objective To investigate the effect of vascular endothelial growth factor receptor-3 (VEGFR-3) on the apoptosis of cervical cancer cells. Methods VEGFR-3 antisense gene eukaryotic expression vector was constructed and transfected to human cervical cancer cell line, Hela cell line by electroporation. The expression level of VEGFR-3 protein before and after transfection was determined by Western Blot and the apoptosis of Hela cell line was observed by Hoechst33258 and flow cytometry. Results After the transfe...