克栓酶抑瘤作用的实验研究

Observed the Inhibitory Tumor Effdct of Batroxobin

摘要: 目的　实验研究克栓酶的抑瘤作用，以期扩展其药用范围，为治疗肿瘤增加药源。方法　采用双层琼脂培养，流式细胞检测，免疫组化图象分析，MTT-ELISA等，以及荷瘤裸鼠整体实验方法观察对人肺癌细胞(PG)与正常人胚内皮细胞增殖抑制作用。结果　证明克栓梅可抑制PG与人胚内皮细胞增殖，具有统计学意义。结论　克栓酶毒性低具有治疗肿瘤的潜能。在肿瘤综合治疗中如何合理联合配伍使用克栓酶值得进一步探讨。

Abstract: Objective To research the Batroxobin DF-521 effect on inhibition of tumor in order to extend its useful range and provide a new drug for curing tumor. Methods To observe the Batroxobin DF-521 effect on human lung cancer cell line PG and normal human embryo vasculo-endothelia cell using double layer agar culture,fluorecetometry,immunohisto chemical immage analysis,MTT-ELISA and inhibitory tumor test with nude mice. Results The Batroxobin was able to inhibit amplification of PG and vasculo-endothelia cells. Conclusion The Batroxobin is a low toxicial drug and has potential in curing tumor.It is worth to explore further how to appropriately use the Batroxobin with other drugs in tumor synthetical treatment.