粪便中大肠脱落细胞提取方法的探讨

Extraction of colorectal exfoliative cells in stool

摘要: 目的　探讨粪便中脱落细胞提取方法, 进一步了解粪便脱落细胞对大肠癌诊断的意义。方法　以 31例均经病理证实为大肠腺癌的患者为研究对象。分别采取免疫磁珠法及改良的淘洗法提取粪便中的脱落细胞。结果　改良的淘洗法与免疫磁珠法对大肠癌的阳性检出率存在差异显著性 (P <0 .0 1)。结论　改良后的粪便中脱落细胞提取方法操作简便、费用低, 易于推广应用。对大肠癌的诊断具有积极意义。

Abstract: Objective To improve the approaches of isolating exfoliated cells from faece,further to analyze the role of exfoliated cells in diagnosis of colorectal cancer. Methods Thirty-one patients with colorectal cancer were entered in our study. Exfoliated cells in the stool of patients were isolated by both methods of elutriation and Immonumagnetic beads (IMB). The method of IMB was performed according to Loktionov, s method. and Elutriation means was Iyengar, s method with slight modification. Result...