Abstract: Objective 　This study aimed to assess the effects of verapamil on signal transduction and proliferation of in vitro meningioma and inquired into the possible mechanisms. Methods 　Spect rofluorometer was used to measure the cytoplasmic free calcium; cell count was used to determine the proliferation of meningioma. Results 　Verapamil in concent ration of 1μmol/ L l can block the proliferation of in vitro meningioma cell induced by FCS, the rate of cell number (vs. cont rol group) is 80 %, FCS induced a quick rise of int racellular free calcium ( P < 0. 01) ; Ca ²⁺ _I was increased to 435. 3 ±34. 9μmol/ L, preincubation with verapamil could inhibit the rise of Ca²⁺ _I, the Ca²⁺ _I >>is 221. 1 ±26. 1 ( P < 0. 01) . Conclusion 　These data indicate that verapamil can stop the influx of Ca²⁺ and hence inhibit the FCS 3/ induced proliferation signal t ransduction.