BPI-1095 对人结肠癌HCT-8 细胞增殖及细胞周期的影响

Effect of NO-ASA on Proliferation and the Cell Cycle in Colon Cancer Cells

摘要: 目的探讨NO-ASA体外对结肠癌细胞增殖、细胞周期的影响。方法采用MTT法检测NO-ASA对体外培养的结肠癌细胞的生长抑制作用;用流式细胞仪检测细胞周期分布。结果 NO-ASA对结肠癌细胞增殖有明显的抑制作用,呈量效和时效依赖关系;对细胞周期也有明显影响,表现为S期细胞比例和G2/M期细胞比例下降,G1期细胞比例升高。结论体外NO-ASA对结肠癌细胞的增殖有明显抑制作用,对细胞周期也有明显影响。

Abstract: Objective 　To investigate the effect of NO-ASA on cell proliferation and cell cycle of colon cancer cells. Methods 　Using MTT method, flow cytometry ( FCM), his effect of BPI-1095 on the HCT-8 of proliferation and cell cycle were studied. Results 　The growth of HCT-8 was inhibited by BPI-1095. The inhibition rate of BPI-1095 on HCT-8 cell proliferation increased as incubation time and BPI-1095 concentration increasing ; the cell ratios of S and G₂ phase decreased, whereas the cell ratio of G₁phase increased with a dose-dependent manner. Conclusion 　BPI-1095 inhibited the growth of colon cancer cells and changed the phase of cell cycle.