OK-432联合IL-2对Lewis肺癌及c-fos蛋白表达的抑制作用

The Inhibitory Effect of OK-432 and IL-2 on Lewis Lung Cancer Growth and c-fos Expression

摘要: 目的　研究OK 4 32与IL 2抑制近交系小鼠C57BL/6Lewis肺癌 (LLC)的生长及c fos蛋白的表达。方法　以C57BL/6荷瘤LLC小鼠为模型, 观察OK 4 32联合IL 2对肿瘤发生、生长转移的影响, 及用抗鼠c fos单克隆抗体进行SABC免疫组化技术半定量测定c fos在Lewis肺癌原发灶中的表达。结果　二者联用对肿瘤体积和重量的抑制作用明显增强 (P <0 0 1), 增强抑瘤率 (P <0 0 1), 肺转移灶数目明显减少。光镜下显示肿瘤灶中出现许多凋亡细胞 ;肿瘤组织电镜观察, 瘤细胞核染色质浓集成块, 在核膜内呈新月形或环形排列, 核膜清楚, 瘤细胞内质网扩张, 线粒体肿胀。c fos在联合用药组与对照组比较明显减少 (P <0 0 1)。结论　OK 4 32与IL 2有明显的协同抗肿瘤活性, 部分原因可能阻抑c fos基因的表达 ;提示OK 4 32联合IL 2可望为临床治疗癌症患者提供一种有效的生物治疗方法。

Abstract: Objective To study the inhibitory effect of OK-432 and IL-2 on the growth of Lewis lung cancer (LLC) bearing C 57 BL/6 mice and c-fos protein expression. Methods As C 57 BL/6 mice bearing LLC model,we observed the effect of OK-432 in combination with IL-2 on the tumor occurrence,growth,metastasis,and the expression of c-fos by using the semiquantitative SABC immunohistochemical method. Results Combination group significantly enhanced inhibitory effect on tumor volume and weight versus control( P <...