Abstract: Objective 　To determine the effect of sequential int ravesical instillation to prevent superficial bladder cancer recurrence and seek ideal methods of int ravesical instillation. Methods 　Four-five patients (34 men, 11 women) with superficial bladder cancer, mean age of 56. 1 years ( range 31～80), were randomly divided into three groups. A group received a single int ravesical instillation of epirubicin as control, B group and C group received different sequential int ravesical instillation of hydroxycamptothecine ( HCPT) and mitomycin Cn (MMC) individually. Results 　The mean follow-up are 26. 36 months, 36. 73 months and 24. 31 months for A, B, C groups. The recur rates are 54. 5 % ( 6/ 11 ), 27. 7 % ( 5/ 18 ), 6. 25 %(1/ 16) individually. Conclusion 　The result s showed that sequential int ravesical with HCPT and MMC in reason maybe have better effect s than a single int ravesical instillation of epirubicin.