外源性p53基因增加卵巢癌细胞对顺铂的敏感性

Enhanced sensitivity of human ovarian cancer cells to cisplatin by exogenous p53 gene

摘要: 目的探讨外源性ｐ５３基因转染对人卵巢癌细胞株的化疗敏感性的影响。方法用脂质体介导的转染技术，将人野生型ｐ５３基因的真核表达载体导入不表达ｐ５３的卵巢癌ＳＫＯＶ－３细胞中，经Ｇ４１８筛选，Ｎｏｒｔｈｅｒｎｂｌｏｔ及Ｗｅｓｔｅｒｎｂｌｏｔ鉴定后，观察其对顺铂作用后的ＳＫＯＶ－３细胞的集落形成及凋亡的影响。结果外源性Ｐ５３基因在转染细胞中有效表达，并增强了顺铂对ＳＫＯＶ－３细胞集落形成的抑制作用及促进了顺铂诱导的细胞凋亡。结论外源性ｐ５３基因能增加卵巢癌细胞对顺铂的敏感性，两者联合作用能更大程度地杀灭肿瘤细胞。

Abstract: Objective To explore the effects of exogenous p53 gene on chemosensitivity of human ovarian cancer cell line SKOV-3. Methods human wild-type p53 gene eukaryotic expression vector was introduced into SKOV-3 cells by lipofectin-mediated gene transfection and the expression of p53 gene in them was de- tected by Northern blot and Western blot. The effects of cisplatin to the transfected cells was observed by colony formation and apoptosis. Results The exogenous p53 gene was e...